Molecular Biologist Sample Resume


Molecular Biologist Sample Resume

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Complete Address
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RESEARCH SCIENTIST

QUALIFICATIONS PROFILE

Technically sophisticated, versatile, and highly talented research scientist offering substantial experience in cell biology, molecular biology, and biochemistry techniques, complemented with solid educational foundation. Exhibit familiarity with different biological systems ranging from bacteria to protozoan parasites, to mammalian cells, including animal models of disease. Equipped with strong background in working with nucleic acids and proteins, including assay development and optimization. Known for developing and improving assays and methods of interests. Accustomed to mentoring interns, technicians, and medical students as well as working with international scientists. Possess a keen eye for detail with proven capability to multitask in a competitive and challenging environment. Multilingual in English, Hindi, and Marathi.

ADDITIONAL COMPETENCIES

MOLECULAR BIOLOGY:
Cloning and mutagenesis methods; construction of vectors; cell-based and in vitro assays; qRT-PCR; RNAi; genomic library; yeast two hybrid; PFGE; ChIP; Southern/ Northern blots

BIOCHEMISTRY:
Western; ELISA; Protein expression and characterization using E. coli and mammalian hosts; FPLC (AKTA); gel shifts; enzymatic assays; pull down and immunoprecipitation

CELL BIOLOGY:
Mammalian cell cultures and transfections; generation of stable cell lines; reporter assays; generation of transgenic mice and genotyping; animal models of disease (mouse laparotomy); isolation of DNA/ RNA from infected tissues; Immunohistochemistry

INFECTIOUS DISEASES:
Aseptic techniques; microbial cultures; BSL-2 laboratory practices; extraction of DNA from infectious tissues; diagnostic PCRs

 EMPLOYMENT HISTORY

RESEARCH SCIENTIST | 2007–PRESENT
ABC UNIVERSITY, DIVISION OF INFECTIOUS DISEASES, CHARLOTTESVILLE, VA, USA

  • Expertly manage one of the projects, which optimize the immunogenicity and protective efficacy of the vaccine against amebiasis using mouse models and subsequently to be used for human trials
  • Initiated the development of molecular tools for Entamoeba histolytica
  • Drive all efforts to develop the first negative selection marker for such pathogen, a plasmid expression vector and presently developing a gene replacement system
  • Determine numerous transcription factors as potential vaccine candidates
  • Instrumental in maintaining radiation and chemical safety records for the laboratory
  • Provide strategic guidance to undergraduate/medical students on their research projects for them to develop theoretical and practical skills for future career growth
  • Deliver informative presentations pertaining to good effects of amebiasis vaccine:
    • It can save millions of children from growth defects due to repeated infection, especially in the third world countries
    • It is readily available and a potential bioterrorism agent, which have a positive impact not only on children health but on anti-terrorism defense capabilities

RESEARCH ASSOCIATE | 2004–2007
ABC UNIVERSITY, DEPARTMENT OF PATHOLOGY, CHARLOTTESVILLE, VA, USA

  • Conducted comprehensive research on Transcriptional Regulation during Spermatogenesis, using a potential candidate gene for male contraceptive vaccine as classified by WHO
  • Played a vital role for generating transgenic mice, as well as identifying the mechanism of germ cell-specific gene expression, which would cause serious diseases such as cancer
  • Rendered high level of assistance in building a laboratory, including all facets of planning to reagent ordering
  • Assumed full responsibility in writing a small foundation grant

POSTDOCTORAL FELLOW | 2001–2004
CDE UNIVERSITY, DURHAM, NC
DEPARTMENT OF BIOCHEMISTRY, MEDICAL UNIVERSITY OF SOUTH CAROLINA, CHARLESTON, SC, USA

  • Applied dynamic leadership talents in managing a project which determine the Mechanism of the Initiation of DNA Replication
  • Utilized highly purified proteins and performed vitro assays to reconstitute a cell-free DNA replication system for the plasmid R6K
  • Examined mechanisms regulating plasmid copy number which has a potential application in large-scale vector preparations for gene therapy

GRADUATE STUDENT | 1994–2000
EFG UNIVERSITY, SCHOOL OF BIOTECHNOLOGY, MADURAI, INDIA

  • Completed the dissertation entitled “Genetic Instability in Streptomyces”
  • Designed physical maps using Pulsed Field Gel electrophoresis (PFGE)
  • Meticulously evaluated the secondary metabolite production by unstable mutants with possible application in strain development and stabilization in fermentation industries
EDUCATION

DOCTOR OF PHILOSOPHY (PHD) IN BIOTECHNOLOGY | EFG University, Madurai, India (Certificate of Equivalency available): 2002
MASTER OF SCIENCE (MS) IN MICROBIOLOGY | GHI University, Maharashtra, India: 1993
BACHELOR OF SCIENCE (BS) IN MICROBIOLOGY |GHI University, Maharashtra, India: 1991

MAJOR ACHIEVEMENTS

  • Travel fellowship from European Molecular Biology Organization (EMBO): 2009
  • Larry Ewing Travel Award from the Society for the Study of Reproduction: 2007
  • National level fellowship from the Government of India: 1994–1999
  • Rank certificate in recognition of the highest academic achievement from University of Pune: 1993

PROFESSIONAL AFFILIATION

  • Member, The American Society for Biochemistry and Molecular Biology (ASBMB)

TECHNICAL PROFICIENCY

  • Microsoft Office Suite, ImageQuant, Fundamental Bioinformatics Tools, and Vector NTI

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