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RESEARCH SCIENTIST
Seasoned, intuitive, and dynamic scientist specializing in Biochemistry and Protein Chemistry, offering strong analytical and problem-solving capabilities, keen attention to details, and professional work habits. Equipped with extensive postdoctoral experience in cancer research and medicinal chemistry. Exemplify proven expertise in protein/antibody purification and protein characterization using mass spectrometry, proteomics sample preparations, bioassays, bioinfomatics, confocal imaging, and molecular biology. Accustomed to deliberating with a team of colleagues to brainstorm on topics crucial in improving overall productivity. Prolific writer with strong qualifications in writing grants, manuscripts, and technical reports. Proven publication record in peer review journals. Demonstrate exceptional planning, prioritizing, and goal-setting skills; constantly contribute in achieving positive outcomes and in meeting vision. Areas of expertise include:
- Exemplify proven success in conducting protein expression in insect, mammalian, and bacterial systems as well as labeling and purification of protein/antibody using gel filtration, ion exchange, hydrophobic, and affinity chromatography with AKTA FPLC system and HPLC
- Skilled at performing LCMS (ion trap, nano-LC-LTQ and nano-LC-LTQ-FTICR), confocal, and multiphoton microscope imaging, coupled with expertise in operating surface plasmon resonance (SPR, BiaCore), spectrofluorimeter, and plate readers (UV and Fluorescence)
- Results-driven, solid team player, and trustworthy professional with strong ability in peptide/protein characterization using mass spectrometry and proteomics database search engines, both completed in fiercely competitive and fast-paced environment
- Proficient in preparing samples for proteomics using 1-D, 2-D gel electrophoresis, tryptic digestions, and western blotting
- Offer unyielding expertise in molecular biology (PCR, construct preparation and cloning), qPCR, siRNA and isolation, and purification of DNA and RNA
- Possess in-depth knowledge of high-throughput assay development, including immunoassays and enzyme assays, cell-based real-time activity assays, fluorescence ligand binding assays, mass spectrometric assays, and radioligand binding assays
- Proven success in kinetic analysis for protein interaction with surface plasmon resonance (SPR, Biacore) and fluorescence ligand binding assays
- Acquire thorough knowledge of mammalian cell culture and transfection
- Adept in confocal and multiphoton microscope imaging using live cell and fixed cells (immunofluorescence)
- Expertly dealt with estrogen, nitric oxide, eicosanoid, apoptotic, and chemoprevention signaling pathways
- Display technical skills in MassMatrix, Mascot, PyMol, Microsoft Office Suite, ChemDraw, and Sigma plot
PROFESSIONAL EXPERIENCE
DEPARTMENT OF BIOLOGICAL CHEMISTRY, UNIVERSITY OF CALIFORNIA ▪ LOS ANGELES, CA
POSTDOCTORAL RESEARCH ASSOCIATE | 2018-PRESENT
- Handle a research project that focus on understanding the mechanism of mitochondrial fission and fusion using proteomics approaches
- Perform immunoassays, gene knockout using siRNA, identification of protein binding partners using mass spectrometry and confocal microscopy, as well as production and purification of monoclonal antibodies for proteases
- Provide technical support and offer research ideas to all graduate students and junior postdoctoral fellows for their projects
- Successfully completing on one-year fellowship from innovative research in Parkinson’s disease
DEPARTMENT OF MEDICINAL CHEMISTRY AND PHARMACOGNOSY, UNIVERSITY OF ILLINOIS ▪ CHICAGO, IL
POSTDOCTORAL RESEARCH ASSOCIATE | 2016-2018
- Recognized and quantified protein S-nitrosation through novel mass spectrometric technique and mapping cysteome for protein nitrosation
- Rendered keen oversight in the development of novel high-throughput immunoassays, fluorescence assays, and mass spectrometric assays for screening protease inhibitors.
- Conducted thorough research and analysis of carcinogenic and chemo preventive effects of estrogen metabolites and novel selective estrogen receptor modulator (SERM) drugs using fluorescence cell-based assays
- Guided and educated graduate/undergraduate students regarding timely and effective completion of individually assigned projects. Contributed in developing grant proposals by offering several research ideas and constructing other parts of the grants
- Conceptualized, developed, and published more than five projects in two years and issued more than seven papers in peer review journals
DEPARTMENT OF CHEMISTRY, UNIVERSITY OF ILLINOIS ▪ CHICAGO, IL
RESEARCH/TEACHING ASSISTANT | 2010-2015
- Worked on a doctoral thesis research, studying the membrane binding and activation mechanisms of Phospholipase A2 and 5-Lipoxygenase enzymes which required protein expression and purification using both bacterial and insect cell lines, protein refolding/engineering, enzyme activity assays, and DNA mutagenesis
- Expertly utilized complex laboratory equipment, such as confocal and multiphoton microscopes, surface plasmon resonance (SPR, Biacore), and spectroflourimeter
- Completed innovative discovery, including identification of novel protein which can transfer across the plasma membrane that subsequently reduce the cell proliferation, as well as novel discovery of phosphorylation activation mechanism of cytosolic Phospholipase A2
- Demonstrated success in accomplishing more than four projects, which were subsequently published in peer review journals, acknowledging as the main author
EDUCATION
DOCTOR OF PHILOSOPHY IN BIOCHEMISTRY: 2016
University of Illinois ▪ Chicago, Chicago, IL, USA
BACHELOR OF SCIENCE IN CHEMISTRY: 2009
University of Kelaniya ▪ Kelaniya, Sri Lanka
